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Interleukin (IL)-1β and IL-18 play central and detrimental roles in the development of acute lung injury (ALI), and mammalian target of rapamycin (mTOR) is involved in regulating IL-1β and IL-18 production. However, it is not clear whether the mTOR specific inhibitor rapamycin can attenuate lipopolysaccharide (LPS)-induced ALI by modulating IL-1β and IL-18 production. In this study, we found that rapamycin ameliorated LPS-induced ALI by inhibiting NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-mediated IL-1β and IL-18 secretion. Mechanistically, elevated autophagy and decreased nuclear factor (NF)-κB activation were associated with downregulated IL-1β and IL-18. Moreover, rapamycin reduced leukocyte infiltration in the lung tissue and bronchoalveolar lavage fluid (BALF), and contributed to the alleviation of LPS-induced ALI. Consistently, rapamycin also significantly inhibited IL-1β and IL-18 production by RAW264.7 cells via increased autophagy and decreased NF-κB signaling in vitro. Our results demonstrated that rapamycin protects mice against LPS-induced ALI partly by inhibiting the production and secretion of IL-1β and IL-18. mTOR and rapamycin might represent an appropriate therapeutic target and strategy for preventing ALI induced by LPS. 相似文献
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《Neuroscience letters》1998,255(3):167-171
Rats received right dorsal root ganglionectomy (DRGn) to induce autotomy, and were treated with MK-801 and/or left stantia nigra (SN) lesion after DRGn. The behavior was quantified using an autotomy grading scale. All the rats in the control groups manifested autotomy from 4 to 19 days after DRGn and attained the highest autotomy score. The group treated with MK-801 immediately after DRGn showed suppression of the development of autotomy. The groups receiving left SN lesion with 6-hydroxydopamine immediately, 2, or 4 days after DRGn showed similar patterns of autotomy as the control groups. However, when combined with the administration of MK-801 immediately after DRGn, SN lesion done immediately or 2 days after DRGn suppressed the antagonistic effect of MK-801 (P<0.01). When the SN lesion was delayed by 4 days, the suppression effect disappeared. These data suggest that the action of the NMDA receptor antagonist on the autotomy within 4 days after DRGn depend on the integrity of the dopaminergic system. 相似文献
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目的探索双酚A(bisphenol A,BPA)对肝脏脂质代谢的影响。方法﹐采用BPA染毒大鼠体内实验,结合人肝细胞系HL-7702体外染毒实验研究双酚A对肝脏脂质代谢的影响。将28只雄性SD大鼠随机分为4组:对照组及BPA亚急性染毒低[1 mg/(kgd)]、中[5 mg/(kg·d)]和高[25 mg/(kg·d)]剂量组,14d后从大鼠体重、肝脏脏器系数,肝脏病理组织切片、血清生化指标等方面评价BPA对肝脏的毒性效应。将人肝细胞系HL-7702,分为对照组及BPA低(0.16 μmol/L)、中(4 μmol/L)和高(100 μmol/L)浓度处理组,染毒24 h后检测细胞内甘油三酯和总胆固醇(totalcholesterol,TC)含量,活性氧簇(reactive oxygen species,ROS)水平,并通过荧光定量PCR检测脂代谢及氧化应激相关基因的转录水平。结果BPA 14 d亚急性染毒后,大鼠体重和肝脏脏器系数无明显变化,但肝脏病理切片发现BPA染毒可损伤肝脏的组织结构。血清生化指标中总胆汁酸在高剂量组显著降低,为(4.75±0.33)μmol/L;肌酐在中﹑高剂量组显著升高,分别为(18.00±0.76)和(18.83±0.75)μmol/L;TC,高密度脂蛋白胆固醇和低密度脂蛋白胆固醇在中,高剂量组均显著降低(P<0.05),分别为(1.44±0.10)、(1.14±0.10)mmol/L,(0.84±0.04)、(0.63±0.07)mmol/L和(0.21±0.04),(0.16±0.05)mmol/L。BPA染毒可使人肝细胞系HL-7702细胞内TC含量显著降低(P<0.05),ROS水平显著增加;PPARα及FABP1转录水平在高浓度处理组中显著升高;SOD1在中,高浓度处理组中均显著降低(P<0.05)。结论BPA可能通过诱导肝脏细胞内产生过量的ROS,导致肝脏损伤和体内脂质代谢紊乱,从而表现出肝脏毒性。 相似文献
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BackgroundAcute graft-versus-host disease (aGVHD) is a complication of allogeneic hematopoietic stem cell transplantation. Ras-related protein 1A (RAP1A) has been recently identified as a novel oncoprotein in several human malignancies. However, its specific role in aGVHD remains unclear.ObjectiveTo study the role of RAP1A in the pathogenesis of aGVHD.Material and methodsStudy participants included six patients with grade 2–4 aGVHD, 13 patients with grade 1 aGVHD, 11 patients without aGVHD, and 12 healthy people. The expression level of RAP1A in whole cells was detected by western blot and qRT-PCR. The proportions of CD4+CD25+FoxP3+ Treg cells (T regulatory cells) and the expression of RAP1A in Treg cells in peripheral blood mononuclear cells (PBMCs) were detected by flow cytometry and the levels of related cytokines in the serum was detected by cytometric bead array.ResultsWe found the level of RAP1A was higher in patients than in healthy individuals. A negative correlation was noted between RAP1A and the number of Treg cells. In addition, the level of IL-10 in patients with grade 2–4 aGVHD was significantly lower than that in healthy donors, however, the level of TNF-ɑ in patients with grade 2–4 aGVHD was higher. Furthermore, we found a negative correlation between levels of IL-10 and RAP1A, and a positive correlation between TNF-ɑ and RAP1A.ConclusionThe expression of RAP1A in patients with aGVHD was significantly increased, and shows potential as a target for the prevention and treatment of aGVHD. 相似文献
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